Ion channels are integral membrane proteins that act, via electrochemical signaling, in cellular communication in the nervous system. Acting as selective filters, they participate in a series of biological processes important for the homeostasis of the organism. In neurons, by amplifying small variations in membrane potential, voltage-sensitive ion channels promote the initiation and propagation of action potentials. The relevance of these channels for the functioning of the neurons make them specific targets for many chemical compounds with pharmacological importance. Even more interesting is the observation that a single amino acid change in their structure can generate a series of pathological phenotypes. Multiple complementary techniques are used in LaNEF to understand the gating mechanism of voltage-sensitive ion channels, their association with some pathological conditions like periodic paralysis, and their pharmacological properties and modulation by lipids such as endocannabinoid ligands, cholesterol, and phospholipids.